Signalling
Part:BBa_K5444000
Designed by: Ihsiu Huang Group: iGEM24_OKstate (2024-09-27)
DsbA signaling peptide (Sec secretion)
This ORF encodes for the DsbA signaling peptide. It allows any protein fused to the peptide to be translocated via the E. coli Sec secretion system. We are using it to tag our Gp3 and Gp8 phage proteins as part of the phagemid construct. The signaling peptide will allow the chimeric Gp3 and Gp8 to be secreted and later incorporated into the engineered M13 phage.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
biology | E. coli |
signalling_molecule | DsbA signaling peptide |